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. 2016;27(1):17-8.
doi: 10.1294/jes.27.17. Epub 2016 Apr 12.

Identification of Immunodiagnostic Antigens for Cerebrospinal Filariasis in Horses by Western Blot Analysis

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Free PMC article

Identification of Immunodiagnostic Antigens for Cerebrospinal Filariasis in Horses by Western Blot Analysis

Masataka Takesue et al. J Equine Sci. .
Free PMC article

Abstract

In the present study, the serum and cerebrospinal fluid of horses diagnosed with Setaria digitata cerebrospinal filariasis were analyzed by western blot. The results revealed S. digitata protein bands measuring 65, 34, 22, and 18 kDa in molecular weight. In particular, the 18 kDa band is a possible candidate for clinical immunodiagnosis on the basis of western blot findings.

Keywords: Setaria digitata; cerebrospinal filariasis; western blot.

Figures

Fig. 1.
Fig. 1.
Setaria digitata proteins in horse serum and cerebrospinal fluid (CSF) analyzed using western blotting. Lane 1, before inoculation; lane 2, after inoculation; lanes 3–5, equine cerebrospinal filariasis; lanes 6–8, protozoal encephalitis; and lane 9, spinal stenosis.

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