Monitoring the transport and phosphorylation of 2-deoxy-D-glucose in tumor cells in vivo and in vitro by 13C nuclear magnetic resonance spectroscopy

FEBS Lett. 1989 Apr 10;247(1):86-90. doi: 10.1016/0014-5793(89)81246-3.

Abstract

We describe the use of 2-deoxy-D-[6-13C]glucose to follow simultaneously, by 13C NMR, both transport and phosphorylation to its 6-phosphate form, in MCF-7 breast cancer cells in vitro and in vivo in subcutaneous tumors in nude mice.

MeSH terms

  • Animals
  • Biological Transport
  • Breast Neoplasms / metabolism*
  • Deoxy Sugars / metabolism*
  • Deoxyglucose / metabolism*
  • Glucose-6-Phosphate* / analogs & derivatives*
  • Glucosephosphates / metabolism
  • Humans
  • Kinetics
  • Magnetic Resonance Spectroscopy*
  • Mice
  • Mice, Nude
  • Neoplasm Transplantation
  • Neoplasms, Experimental / metabolism
  • Phosphorylation
  • Tumor Cells, Cultured

Substances

  • Deoxy Sugars
  • Glucosephosphates
  • 2-deoxyglucose-6-phosphate
  • Glucose-6-Phosphate
  • Deoxyglucose