The mRNA Encoding Elongation Factor 1-alpha (EF-1 Alpha) Is a Major Transcript at the Midblastula Transition in Xenopus

Dev Biol. 1989 May;133(1):93-100. doi: 10.1016/0012-1606(89)90300-x.

Abstract

A Xenopus laevis gastrula cDNA library has been screened in order to identify sequences that are expressed early in development. We find that the mRNA encoding translation elongation factor 1-alpha (EF-1 alpha) is synthesized in very large amounts in the early embryo. Transcription of EF-1 alpha mRNA commences at the midblastula transition (MBT), and new EF-1 alpha protein is synthesized very soon after this, as determined by the association of EF-1 alpha mRNA with polysomes. The nucleotide sequence of a full-length EF-1 alpha cDNA clone and the deduced amino acid sequence of Xenopus EF-1 alpha protein are presented.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blastocyst / metabolism*
  • DNA / genetics
  • DNA Probes
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Peptide Elongation Factor 1
  • Peptide Elongation Factors / genetics*
  • Polyribosomes / metabolism
  • RNA Probes
  • RNA, Messenger / genetics*
  • RNA, Messenger / metabolism
  • Transcription, Genetic*
  • Xenopus laevis / embryology

Substances

  • DNA Probes
  • Peptide Elongation Factor 1
  • Peptide Elongation Factors
  • RNA Probes
  • RNA, Messenger
  • DNA

Associated data

  • GENBANK/M25504