Adipose stromal cells improve healing of vocal fold scar: Morphological and functional evidences

Laryngoscope. 2016 Aug;126(8):E278-85. doi: 10.1002/lary.25867. Epub 2016 Apr 14.


Objectives/hypothesis: Adipose derived stromal cells (ASCs) are abundant and easy to prepare. Such cells may be useful for treating severe vocal disturbance caused by acute vocal fold scars.

Study design: Prospective animal experiments with controls.

Methods: Twenty New-Zealand white rabbits were used in the present study. We evaluated vocal fold healing, with or without injection of autologous ASCs, after acute scarring. A defined lesion was created and the ASCs were immediately injected. Vocal fold regeneration was evaluated histomorphometrically and via viscoelastic analysis using an electrodynamic shaker.

Results: Six weeks after ASC injection, vocal folds exhibited significantly less inflammation than control folds (P < 0.005). In addition, hypertrophy of the lamina propria and fibrosis were significantly reduced upon ASC injection (P < 0.02). The decrease in viscoelastic parameters was less important in the ASC injected group compared to the noninjected group (P = 0.08).

Conclusion: Injection of autologous ASCs improved vocal fold healing in our preclinical model. Further studies are needed, but this method may be useful in humans.

Level of evidence: NA. Laryngoscope, 126:E278-E285, 2016.

Keywords: Wound healing; adipose stromal cells; biomechanical analysis; cytotherapy; vocal fold scar.

MeSH terms

  • Adipose Tissue / cytology*
  • Animals
  • Cicatrix / pathology
  • Cicatrix / surgery*
  • Laryngeal Diseases / pathology
  • Laryngeal Diseases / surgery*
  • Prospective Studies
  • Rabbits
  • Stromal Cells / transplantation*
  • Vocal Cords*