Allergy Vaccines Using a Mycobacterium-Secreted Antigen, Ag85B, and an IL-4 Antagonist

Methods Mol Biol. 2016;1403:723-38. doi: 10.1007/978-1-4939-3387-7_41.

Abstract

In recent decades, the prevalence of allergic diseases, including bronchial asthma, airway hypersensitivity, hay fever, and atopic dermatitis, has been increasing in the industrialized world, and effective treatments probably require manipulating the inflammatory response to pathogenic allergens. T helper (Th) 2 cells are thought to play a crucial role in the initiation, progression, and persistence of allergic responses in association with production of interleukin (IL)-4, IL-5, and IL-13. Therefore, a strategy of a shift from Th2- to Th1-type immune response may be valuable in the prophylaxis and management of allergic diseases. It is also necessary to develop prophylactic and therapeutic treatment that induces homeostatic functions in the multifaceted allergic environment, because various factors including innate and adaptive immunity, mucosal immune response, and functional and structural maintenance of local tissue might be involved in the pathogenesis of allergic disorders. We review herein recent findings related to the curative effect for mouse models of asthma and atopic dermatitis using DNA-, virus-, and protein-based vaccines of a Mycobacterium secretion antigen, Ag85B, and a plasmid encoding cDNA of antagonistic IL-4 mutant.

Keywords: Ag85B; Asthma; Atopic dermatitis; DNA; HPIV2; IFN-γ; IL-4; Mutant IL-4; Recombinant protein; Th1; Th2; Vaccine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyltransferases / genetics
  • Acyltransferases / immunology*
  • Acyltransferases / pharmacology*
  • Animals
  • Antigens, Bacterial / genetics
  • Antigens, Bacterial / immunology*
  • Antigens, Bacterial / pharmacology*
  • Asthma / prevention & control*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / immunology*
  • Bacterial Proteins / pharmacology*
  • Dermatitis, Atopic / prevention & control*
  • Female
  • Interleukin-4 / antagonists & inhibitors*
  • Mice
  • Mutation
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Recombinant Proteins / pharmacology
  • Vaccines / immunology*
  • Vaccines, DNA / genetics
  • Vaccines, DNA / immunology
  • Vaccines, DNA / pharmacology

Substances

  • Antigens, Bacterial
  • Bacterial Proteins
  • Recombinant Proteins
  • Vaccines
  • Vaccines, DNA
  • Interleukin-4
  • Acyltransferases
  • antigen 85B, Mycobacterium tuberculosis