Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Filters applied. Clear all
. 2016 Apr 15;6:22958.
doi: 10.1038/srep22958.

Bacterial Communities of the Cotton Aphid Aphis Gossypii Associated With Bt Cotton in Northern China

Affiliations
Free PMC article

Bacterial Communities of the Cotton Aphid Aphis Gossypii Associated With Bt Cotton in Northern China

Yao Zhao et al. Sci Rep. .
Free PMC article

Abstract

Aphids are infected with a wide variety of endosymbionts that can confer ecologically relevant traits. However, the bacterial communities of most aphid species are still poorly characterized. This study investigated the bacterial diversity of the cotton aphid Aphis gossypii associated with Bt cotton in northern China by targeting the V4 region of the 16S rDNA using the Illumina MiSeq platform. Our sequencing data revealed that bacterial communities of A. gossypii were generally dominated by the primary symbiont Buchnera, together with the facultative symbionts Arsenophonus and Hamiltonella. To our knowledge, this is the first report documenting the facultative symbiont Hamiltonella in A. gossypii. Moreover, the bacterial community structure was similar within aphids from the same province, but distinct among those from different provinces. The taxonomic diversity of the bacterial community is greater in Hebei Province compared with in samples from Henan and Shandong Provinces. The selection pressure exerted by the different geographical locations could explain the differences found among the various provinces. These findings broaden our understanding of the interactions among aphids, endosymbionts and their environments, and provide clues to develop potential biocontrol techniques against this cotton aphid.

Figures

Figure 1
Figure 1. Heat maps showing the relative abundance and distribution of A. gossypii bacterial family in three provinces of northern China.
The color code indicates relative abundance, ranging from blue (low abundance) to yellow to red (high abundance).
Figure 2
Figure 2. Comparison of bacterial community structures in A. gossypii samples in three provinces of northern China.
Unweighted and weighted UniFrac metrics were used to determine pairwise distances between all samples.
Figure 3
Figure 3. Sampling locations of A. gossypii in three provinces of northern China.
Henan Province: Zhoukou(Zk), Xuchang(Xc), Shangqiu(Sq). Hebei Province: Cangzhou(Cz), Hengshui(Hs), Handan(Hd). Shandong Province: Jining(Jn), Bingzhou(Bz), Heze(Hz), Dezhou(Dz). The map is created with ArcGIS 10.2 (http://www.arcgis.com/features/).

Similar articles

See all similar articles

Cited by 7 articles

See all "Cited by" articles

References

    1. Wernegreen J. J. Mutualism meltdown in insects: bacteria constrain thermal adaptation. Curr. Opin. Microbiol. 15, 255–262 (2012). - PMC - PubMed
    1. Colman D. R., Toolson E. C. & Takacs-Vesbach C. Do diet and taxonomy influence insect gut bacterial communities? Mol. Ecol. 21, 5124–5137 (2012). - PubMed
    1. Jing X. et al. The bacterial communities in plant phloem-sap-feeding insects. Mol. Ecol. 23, 1433–1444 (2014). - PubMed
    1. Oliver K. M., Degnan P. H., Burke G. R. & Moran N. A. Facultative symbionts in aphids and the horizontal transfer of ecologically important traits. Annu. Rev. Entomol. 55, 247–266 (2010). - PubMed
    1. Buchner P. Endosymbiosis of Animals with Plant Microorganisms (ed. Buchner P.) 909 pp. (Interscience, New York, 1965).

Publication types

Substances

Feedback