Water-insoluble thin films from palmitoyl hyaluronan with tunable properties

Carbohydr Polym. 2016 Jun 25:144:68-75. doi: 10.1016/j.carbpol.2016.02.027. Epub 2016 Feb 10.


Hyaluronan (HA) films exhibit properties suitable for various biomedical applications, but the solubility of HA limits their use in aqueous environments. Therefore, we developed water insoluble films based on palmitoyl esters of HA (pHA). Films were prepared from pHA samples with various degrees of substitution (DS) and molecular weights and their mechanical properties and swelling were characterized. Additionally, scanning electron microscopy and atomic force microscopy were used for visualization. Despite being prepared by solution casting, the films had a very smooth surface and were homogeneous in thickness. The film properties were in accordance with the polymer DS and molecular weight, enabling to tailor them for future applications by choosing a suitable pHA material. The behavior of the films toward cells was assessed in vitro. All films were non-cytotoxic and showed no adhesion of cells. These results show that the developed films are suitable candidates for various biomedical applications such as tissue engineering or wound healing.

Keywords: Palmitoyl hyaluronan; Solution casting; Swelling; Tensile properties; Thin film.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acylation
  • Animals
  • Cell Adhesion / drug effects
  • Cell Line, Tumor
  • Flow Cytometry
  • Humans
  • Hyaluronic Acid / analogs & derivatives
  • Hyaluronic Acid / chemistry*
  • Hyaluronic Acid / toxicity
  • Mice
  • Microscopy, Atomic Force
  • Microscopy, Electron, Scanning
  • Palmitates / chemistry*
  • Palmitates / toxicity
  • Solubility
  • Swiss 3T3 Cells
  • Tensile Strength
  • Water / chemistry


  • Palmitates
  • Water
  • Hyaluronic Acid