Distinct metabolic effects of resveratrol on lipogenesis markers in mice adipose tissue treated with high-polyunsaturated fat and high-protein diets

Life Sci. 2016 May 15:153:66-73. doi: 10.1016/j.lfs.2016.04.014. Epub 2016 Apr 14.


Objective: A healthy diet is essential for the prevention of metabolic syndrome. The present study evaluated the effect of resveratrol associated with high-polyunsaturated fat and high-protein diets on expression of adipogenic and lipogenic genes.

Research methods & procedures: FVB/N mice were divided into 6 groups (n=7 each) and fed with experimental diets for 60days: standard (ST), high-fat diet (HFD), and high-protein diet (HPD), with and without resveratrol (RSV) (4g/kg diet). The body weight, food intake, energy intake (kcal), and blood parameters (HDL-C, total cholesterol, glucose, and triglyceride levels) were assessed. Real-time PCR was performed to analyze the expression of adipogenesis and lipogenesis markers: PPARγ, SREBP-1c, ACC and FAS in samples from perigonadal adipose tissue.

Results: In the HPD+RSV group, resveratrol decreased body weight, body adiposity, adipose tissue weight, adipocyte area, total cholesterol, ACC and FAS expression, and increased HDL-cholesterol in comparison to HPD. In the HPD group there was a decrease in adipocyte area, as well as PPARγ, SREBP-1c and ACC expression in comparison to ST. While in HFD+RSV, resveratrol decreased levels of total cholesterol in comparison to HFD. In the HFD group there was decrease in body weight, and PPARγ, SREBP-1c and ACC expression in comparison to ST.

Conclusions: The obtained results show that resveratrol decreases lipogenesis markers and metabolic parameters in the setting of a high-protein diet. Moreover, resveratrol decreased total cholesterol in both diets. These results point to the increased potential of resveratrol use in prevention of metabolic syndrome, acting on different dietary compositions.

Keywords: Adipogenesis; Cholesterol; Diet; Lipogenesis; Resveratrol; Resveratrol (PubChem CID: 445154).

MeSH terms

  • Adipogenesis / genetics
  • Adipose Tissue / drug effects*
  • Animals
  • Body Composition
  • Dietary Fats, Unsaturated / administration & dosage*
  • Dietary Proteins / administration & dosage*
  • Fatty Acids, Unsaturated / administration & dosage*
  • Female
  • Lipogenesis / drug effects*
  • Mice
  • Resveratrol
  • Stilbenes / pharmacology*


  • Dietary Fats, Unsaturated
  • Dietary Proteins
  • Fatty Acids, Unsaturated
  • Stilbenes
  • Resveratrol