Sequencing and molecular modeling identifies candidate members of Caliciviridae family in bats

Infect Genet Evol. 2016 Jul;41:227-232. doi: 10.1016/j.meegid.2016.04.004. Epub 2016 Apr 13.

Abstract

Emerging viral diseases represent an ongoing challenge for globalized world and bats constitute an immense, partially explored, reservoir of potentially zoonotic viruses. Caliciviruses are important human and animal pathogens and, as observed for human noroviruses, they may impact on human health on a global scale. By screening fecal samples of bats in Hungary, calicivirus RNA was identified in the samples of Myotis daubentonii and Eptesicus serotinus bats. In order to characterize more in detail the bat caliciviruses, large portions of the genome sequence of the viruses were determined. Phylogenetic analyses and molecular modeling identified firmly the two viruses as candidate members within the Caliciviridae family, with one calicivirus strain resembling members of the Sapovirus genus and the other bat calicivirus being more related to porcine caliciviruses of the proposed genus Valovirus. This data serves the effort for detecting reservoir hosts for potential emerging viruses and recognize important evolutionary relationships.

Keywords: Bat; Calicivirus; Daubenton's bat; Europe; Hungary; Protein modeling; Serotine bat; Virus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Caliciviridae / classification
  • Caliciviridae / genetics*
  • Caliciviridae / isolation & purification
  • Caliciviridae Infections / epidemiology*
  • Caliciviridae Infections / transmission
  • Caliciviridae Infections / virology
  • Capsid Proteins / chemistry*
  • Capsid Proteins / genetics
  • Chiroptera / virology*
  • Disease Reservoirs / virology*
  • Feces / virology
  • Gene Expression
  • Genome, Viral*
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Hungary / epidemiology
  • Models, Molecular
  • Phylogeny*
  • Protein Conformation, alpha-Helical
  • Protein Conformation, beta-Strand
  • RNA, Viral / genetics
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Swine

Substances

  • Capsid Proteins
  • RNA, Viral