The diagnostic significance of serum alcohol dehydrogenase isoenzymes and aldehyde dehydrogenase activity in renal cell cancer patients

Exp Mol Pathol. 2016 Jun;100(3):416-20. doi: 10.1016/j.yexmp.2016.04.001. Epub 2016 Apr 14.


Objectives: In previous experiments, we have found an increased level of class I ADH and total ADH activity in RCC tissues. Changes in cancer cells may be reflected by ADH activity in the serum and could thus be helpful for diagnostics of renal cancer. The aim of this study was to investigate a potential role of ADH and ALDH as tumor markers for RCC.

Material and methods: Serum samples were taken from 59 patients with RCC and 52 healthy subjects. Class III and IV of ADH and total ADH activity was measured by the photometric method. For measurement of class I and II ADH and ALDH activity, we employed the fluorometric method.

Results: The total activity of ADH and ADH I was significantly higher in the serum of patients with every stage of RCC compared to healthy subjects. The diagnostics criteria was higher for ADH I than for total ADH activity. The diagnostic sensitivity for ADH I was 73.36%, specificity 85.61%, predictive values of positive and negative results were 79.12 and 75.03% respectively. Area under ROC curve for ADH I was 0.748 and for total ADH 0.689.

Conclusion: The results suggest a potential role of ADH I as a marker for RCC.

Keywords: Alcohol dehydrogenase isoenzymes; Aldehyde dehydrogenase; Cancer markers; Diagnostic utility; Renal cell carcinoma.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Alcohol Dehydrogenase / blood
  • Alcohol Dehydrogenase / metabolism*
  • Aldehyde Dehydrogenase / blood
  • Aldehyde Dehydrogenase / metabolism*
  • Biomarkers, Tumor / blood
  • Carcinoma, Renal Cell / blood
  • Carcinoma, Renal Cell / diagnosis
  • Carcinoma, Renal Cell / enzymology*
  • Female
  • Fluorometry
  • Humans
  • Isoenzymes / blood
  • Isoenzymes / metabolism
  • Kidney Neoplasms / blood
  • Kidney Neoplasms / diagnosis
  • Kidney Neoplasms / enzymology*
  • Male
  • Middle Aged
  • ROC Curve


  • Biomarkers, Tumor
  • Isoenzymes
  • Alcohol Dehydrogenase
  • Aldehyde Dehydrogenase