A Reusable, Compliant, Small Volume Blood Reservoir for In Vitro Hemolysis Testing

Artif Organs. 2017 Feb;41(2):175-178. doi: 10.1111/aor.12724. Epub 2016 Apr 18.


Bench-top in vitro hemolysis testing is a fundamental tool during the design and regulatory safety evaluation of blood-contacting medical devices. While multiple published experimental protocols exist, descriptions of the test loop reservoir remain ambiguous. A critical fixture within the circuit, there is no readily available blood reservoir that ensures thorough mixing and complete air evacuation: two major factors which can affect results. As part of the Food and Drug Administration (FDA) Critical Path Initiative, we developed a three-piece reservoir consisting of a 3D-printed base, a plastic clamp set, and a medical-grade blood bag. This simple, reusable, and cost-effective design was used successfully in the hemolysis assessment of FDA benchmark nozzles and prototype rotary blood pumps, and may be useful as an integral component to any in vitro blood circulation loop.

Keywords: Blood reservoir; Cardiopulmonary bypass; In vitro hemolysis testing; Mechanical circulatory support devices.

MeSH terms

  • Assisted Circulation / adverse effects*
  • Assisted Circulation / instrumentation
  • Cardiopulmonary Bypass / adverse effects*
  • Cardiopulmonary Bypass / instrumentation
  • Equipment Design
  • Erythrocytes / pathology*
  • Hematologic Tests / instrumentation
  • Hemolysis*
  • Humans
  • Hydrodynamics