Light-induced nuclear export reveals rapid dynamics of epigenetic modifications

Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. Epub 2016 Apr 18.

Abstract

We engineered a photoactivatable system for rapidly and reversibly exporting proteins from the nucleus by embedding a nuclear export signal in the LOV2 domain from phototropin 1. Fusing the chromatin modifier Bre1 to the photoswitch, we achieved light-dependent control of histone H2B monoubiquitylation in yeast, revealing fast turnover of the ubiquitin mark. Moreover, this inducible system allowed us to dynamically monitor the status of epigenetic modifications dependent on H2B ubiquitylation.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Cell Nucleus / metabolism*
  • Cell Nucleus / radiation effects*
  • Epigenesis, Genetic / radiation effects*
  • Flavoproteins / chemistry
  • Flavoproteins / genetics
  • Flavoproteins / metabolism*
  • Histones / metabolism*
  • Light*
  • Nuclear Export Signals
  • Phototropins / chemistry
  • Protein Domains
  • Protein Transport / radiation effects
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae / radiation effects
  • Saccharomyces cerevisiae Proteins / metabolism
  • Ubiquitin / metabolism
  • Ubiquitination / radiation effects*

Substances

  • Bre1 protein, S cerevisiae
  • Flavoproteins
  • Histones
  • Nuclear Export Signals
  • Phototropins
  • Saccharomyces cerevisiae Proteins
  • Ubiquitin