Microbiota-based Signature of Gingivitis Treatments: A Randomized Study

Sci Rep. 2016 Apr 20;6:24705. doi: 10.1038/srep24705.


Plaque-induced gingivitis can be alleviated by various treatment regimens. To probe the impacts of various anti-gingivitis treatments on plaque microflora, here a double blinded, randomized controlled trial of 91 adults with moderate gingivitis was designed with two anti-gingivitis regimens: the brush-alone treatment and the brush-plus-rinse treatment. In the later group, more reduction in both Plaque Index (TMQHI) and Gingival Index (mean MGI) at Day 3, Day 11 and Day 27 was evident, and more dramatic changes were found between baseline and other time points for both supragingival plaque microbiota structure and salivary metabonomic profiles. A comparison of plaque microbiota changes was also performed between these two treatments and a third dataset where 50 subjects received regimen of dental scaling. Only Actinobaculum, TM7 and Leptotrichia were consistently reduced by all the three treatments, whereas the different microbial signatures of the three treatments during gingivitis relieve indicate distinct mechanisms of action. Our study suggests that microbiota based signatures can serve as a valuable approach for understanding and potentially comparing the modes of action for clinical treatments and oral-care products in the future.

Publication types

  • Clinical Trial
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Biodiversity
  • Cluster Analysis
  • Dental Plaque / microbiology
  • Dental Plaque Index
  • Female
  • Gingivitis / diagnosis
  • Gingivitis / microbiology*
  • Gingivitis / therapy*
  • Humans
  • Male
  • Metabolome
  • Metabolomics / methods
  • Microbiota* / drug effects
  • Middle Aged
  • Mouthwashes*
  • Saliva / metabolism
  • Toothbrushing*
  • Young Adult


  • Mouthwashes