Do nucleic acids moonlight as molecular chaperones?

Nucleic Acids Res. 2016 Jun 2;44(10):4835-45. doi: 10.1093/nar/gkw291. Epub 2016 Apr 21.


Organisms use molecular chaperones to combat the unfolding and aggregation of proteins. While protein chaperones have been widely studied, here we demonstrate that DNA and RNA exhibit potent chaperone activity in vitro Nucleic acids suppress the aggregation of classic chaperone substrates up to 300-fold more effectively than the protein chaperone GroEL. Additionally, RNA cooperates with the DnaK chaperone system to refold purified luciferase. Our findings reveal a possible new role for nucleic acids within the cell: that nucleic acids directly participate in maintaining proteostasis by preventing protein aggregation.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Chaperonin 60 / metabolism
  • DNA / metabolism*
  • Molecular Chaperones / metabolism
  • Protein Aggregates
  • Protein Denaturation
  • Protein Refolding*
  • RNA / metabolism*


  • Chaperonin 60
  • Molecular Chaperones
  • Protein Aggregates
  • RNA
  • DNA