Purification, characterization and antioxidant activity of polysaccharides from Flammulina velutipes residue

Ying Liu; Bin Zhang; S A Ibrahim; Shuang-Shuang Gao; Hong Yang; Wen Huang

doi:10.1016/j.carbpol.2016.03.020

Purification, characterization and antioxidant activity of polysaccharides from Flammulina velutipes residue

Carbohydr Polym. 2016 Jul 10:145:71-7. doi: 10.1016/j.carbpol.2016.03.020. Epub 2016 Mar 11.

Authors

Ying Liu¹, Bin Zhang², S A Ibrahim³, Shuang-Shuang Gao², Hong Yang², Wen Huang⁴

Affiliations

¹ College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
² College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
³ Department of Family and Consumer Sciences, North Carolina A&T State University, 171 Carver Hall, Greensboro, NC 27411, United States.
⁴ College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. Electronic address: huangwen@mail.hzau.edu.cn.

PMID: 27106153
DOI: 10.1016/j.carbpol.2016.03.020

Abstract

In this study, we isolated polysaccharides from Flammulina velutipes residue (FVRP) using microwave-assisted extraction and then purified the polysaccharides by column chromatography to yield FVRP-1, FVRP-2 and FVRP-3. The structural characteristics of FVRP-1, FVRP-2 and FVRP-3 were investigated, and their antioxidant activities against ABTS(+), DPPH and hydroxyl radicals were also analyzed in vitro. FVRP-1 was found to be neutral and rich in galactose. However, FVRP-2 and FVRP-3 were acidic polysaccharides and were rich in glucose. The average molecular weight of FVRP-1, FVRP-2 and FVRP-3 were 29,930, 62,290, and 36,310Da, respectively. The glycosyl residue of FVRP-1 was an α-type glycosidic linkage, whereas FVRP-2 and FVRP-3 were β-type glycosidic linkages. We found FVRP-1, FVRP-2 and FVRP-3 had strong potential antioxidant activities in the order of FVRP-1<FVRP-3<FVRP-2. Our results thus suggested that FVRPs might be suitable for use as functional foods and as potential therapeutic agents.

Keywords: Antioxidant activity; Characterization; Flammulina velutipes residue; Polysaccharide; Purification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antioxidants / chemistry*
Antioxidants / isolation & purification
Benzothiazoles / chemistry
Biphenyl Compounds / chemistry
Flammulina*
Food Industry
Hydroxyl Radical / chemistry
Industrial Waste
Molecular Weight
Monosaccharides / analysis
Picrates / chemistry
Polysaccharides / chemistry*
Polysaccharides / isolation & purification
Sulfonic Acids / chemistry

Substances

Antioxidants
Benzothiazoles
Biphenyl Compounds
Industrial Waste
Monosaccharides
Picrates
Polysaccharides
Sulfonic Acids
2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
Hydroxyl Radical
1,1-diphenyl-2-picrylhydrazyl