The Effect of a Vegetarian Versus Conventional Hypocaloric Diet on Serum Concentrations of Persistent Organic Pollutants in Patients With Type 2 Diabetes

Nutr Metab Cardiovasc Dis. 2016 May;26(5):430-8. doi: 10.1016/j.numecd.2016.01.008. Epub 2016 Jan 28.

Abstract

Background and aims: The aim of this study was to explore the effect of a vegetarian versus conventional diet on the serum levels of persistent organic pollutants (POPs) in patients with T2D after 12 weeks of dietary intervention and to assess their relationships with metabolic parameters.

Methods and results: Men and women with T2D were randomly assigned to follow either a vegetarian diet without fish or meat (n = 37) or an isocaloric conventional antidiabetic diet (n = 37). Both diets were energy restricted (minus 500 kcal/day). All foods were provided to the participants. At randomization (week 0) and 12 weeks, the meal test was performed to assess the β-cell function and serum levels of 24 POPs. Dioxins and dioxin-like POPs were analyzed by isotope dilution high-resolution gas chromatography (HRGC) and mass spectrometry after cleanup of the silica and carbon columns. Non-dioxin-like POPs were analyzed by gas chromatography with an electron capture detector (GC-ECD). Statistical analyses used were repeated-measures analysis of variance (ANOVA), a multivariate regression model, and Pearson's correlations. We observed a statistically nonsignificant trend toward increases in the serum levels of most POPs in response to both hypocaloric diets with no differences between groups. In the groups combined, the change in serum concentrations of total POPs was correlated to changes in HbA1c (r = +0.34; p < 0.01), fasting plasma glucose (r = +0.41; p < 0.01) levels, and β-cell function measured as insulin secretion at a reference glucose level (r = -0.37; p < 0.01), independent of the changes in body weight and volume of visceral fat.

Conclusion: Short-term hypocaloric vegetarian and conventional diets did not reduce the POP levels, possibly due to mobilization of fat stores. Our findings support the relationship between POPs and diabetes, especially β-cell function.

Trial registration: ClinicalTrials.gov number, NCT00883038, completed.

Keywords: Persistent organic pollutants; Type 2 diabetes; Vegetarian diet.

Publication types

  • Comparative Study
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adiposity
  • Adult
  • Aged
  • Biomarkers / blood
  • Blood Glucose / metabolism
  • Caloric Restriction*
  • Czech Republic
  • Diabetes Mellitus, Type 2 / blood
  • Diabetes Mellitus, Type 2 / diagnosis
  • Diabetes Mellitus, Type 2 / diet therapy*
  • Diabetes Mellitus, Type 2 / physiopathology
  • Diet, Reducing*
  • Diet, Vegetarian*
  • Dioxins / adverse effects
  • Dioxins / blood*
  • Environmental Exposure
  • Environmental Pollutants / adverse effects
  • Environmental Pollutants / blood*
  • Female
  • Food Contamination*
  • Gas Chromatography-Mass Spectrometry
  • Glycated Hemoglobin A / metabolism
  • Humans
  • Insulin / blood
  • Insulin-Secreting Cells / metabolism
  • Intra-Abdominal Fat / metabolism
  • Intra-Abdominal Fat / physiopathology
  • Male
  • Middle Aged
  • Multivariate Analysis
  • Time Factors
  • Treatment Outcome
  • Weight Loss

Substances

  • Biomarkers
  • Blood Glucose
  • Dioxins
  • Environmental Pollutants
  • Glycated Hemoglobin A
  • Insulin
  • hemoglobin A1c protein, human

Associated data

  • ClinicalTrials.gov/NCT00883038