Panax ginseng Adventitious Root Suspension Culture: Protocol for Biomass Production and Analysis of Ginsenosides by High Pressure Liquid Chromatography

Methods Mol Biol. 2016;1391:125-39. doi: 10.1007/978-1-4939-3332-7_9.


Panax ginseng C.A. Meyer (Korean ginseng) is a popular herbal medicine. It has been used in Chinese and Oriental medicines since thousands of years. Ginseng products are generally used as a tonic and an adaptogen to resist the adverse influence of a wide range of physical, chemical and biological factors, and to restore homeostasis. Ginsenosides or ginseng saponins are the principal active ingredients of ginseng. Since ginseng cultivation process is very slow and needs specific environment for field cultivation, cell and tissue cultures are sought as alternatives for the production of ginseng biomass and bioactive compounds. In this chapter, we focus on methods of induction of adventitious roots from ginseng roots, establishment of adventitious root suspension cultures using bioreactors, procedures for processing of adventitious roots, and analysis of ginsenosides by high pressure liquid chromatography.

Keywords: Adventitious roots; Bioreactors; Ginsenoside; Herbal medicine; Panax ginseng; Saponin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomass
  • Bioreactors
  • Chromatography, High Pressure Liquid / methods*
  • Equipment Design
  • Flavonoids / analysis
  • Ginsenosides / analysis*
  • Hydroponics / instrumentation
  • Hydroponics / methods*
  • Panax / chemistry
  • Panax / growth & development*
  • Phenols / analysis
  • Plant Roots / chemistry
  • Plant Roots / growth & development*
  • Plants, Medicinal / chemistry
  • Plants, Medicinal / growth & development


  • Flavonoids
  • Ginsenosides
  • Phenols