N,N-Bis(glycityl)amines as Anti-Cancer Drugs

Bioorg Med Chem. 2016 Sep 1;24(17):3932-3939. doi: 10.1016/j.bmc.2016.04.016. Epub 2016 Apr 9.

Abstract

A series of N,N-bis(glycityl)amines with promising anti-cancer activity were prepared via the reductive amination of pentoses and hexoses, and subsequently screened for their ability to selectively inhibit the growth of cancerous versus non-cancerous cells. For the first time, we show that this class of compounds possesses anti-proliferative activity, and, while the selective killing of brain cancer (LN18) cells versus matched (SVG-P12) cells was modest, several of the amines, including d-arabinitylamine 1a and d-fucitylamine 1g, exhibited low micromolar IC50 values for HL60 cells. Moreover, these two amines showed good selectivity towards HL60 cells when compared to non-cancerous HEK-293 cells. The compounds also showed low micromolar inhibition of the leukaemic cell line, THP-1. The modes of action of amines 1a and 1g were then determined using yeast chemical genetics, whereby it was established that both compounds affect similar but distinct sets of biochemical pathways. Notably purine nucleoside monophosphate biosynthesis was identified as an enriched mechanism. The rapid synthesis of the amines and their unique mode of action thus make them attractive targets for further development as anti-cancer drugs.

Keywords: Amine; Anti-cancer; Carbohydrate; Chemical genetics; Synthesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Sugars / chemical synthesis
  • Amino Sugars / pharmacology*
  • Antineoplastic Agents / chemical synthesis
  • Antineoplastic Agents / pharmacology*
  • Cell Line, Tumor
  • DEAD-box RNA Helicases / genetics
  • DEAD-box RNA Helicases / metabolism
  • Doxorubicin / pharmacology
  • HEK293 Cells
  • Humans
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism
  • Structure-Activity Relationship
  • Sugar Alcohols / chemical synthesis
  • Sugar Alcohols / pharmacology*
  • tRNA Methyltransferases / genetics
  • tRNA Methyltransferases / metabolism

Substances

  • Amino Sugars
  • Antineoplastic Agents
  • Saccharomyces cerevisiae Proteins
  • Sugar Alcohols
  • Doxorubicin
  • tRNA Methyltransferases
  • DBP7 protein, S cerevisiae
  • DEAD-box RNA Helicases