Biodiesel Production from Chlorella protothecoides Oil by Microwave-Assisted Transesterification

Int J Mol Sci. 2016 Apr 22;17(4):579. doi: 10.3390/ijms17040579.


In this study, biodiesel production from microalgal oil by microwave-assisted transesterification was carried out to investigate its efficiency. Transesterification reactions were performed by using Chlorella protothecoides oil as feedstock, methanol, and potassium hydroxide as the catalyst. Methanol:oil ratio, reaction time and catalyst:oil ratio were investigated as process parameters affected methyl ester yield. 9:1 methanol/oil molar ratio, 1.5% KOH catalyst/oil ratio and 10 min were optimum values for the highest fatty acid methyl ester yield.

Keywords: Chlorella protothecoides; algal biodiesel; methyl ester; microwave; transesterification.

MeSH terms

  • Biofuels*
  • Catalysis
  • Chlorella / chemistry*
  • Chlorella / metabolism
  • Esterification
  • Fatty Acids / analysis
  • Hydroxides / chemistry
  • Methanol / chemistry
  • Microwaves*
  • Plant Oils / chemistry*
  • Plant Oils / metabolism
  • Potassium Compounds / chemistry


  • Biofuels
  • Fatty Acids
  • Hydroxides
  • Plant Oils
  • Potassium Compounds
  • potassium hydroxide
  • Methanol