irCLIP platform for efficient characterization of protein-RNA interactions

Nat Methods. 2016 Jun;13(6):489-92. doi: 10.1038/nmeth.3840. Epub 2016 Apr 25.


The complexity of transcriptome-wide protein-RNA interaction networks is incompletely understood. While emerging studies are greatly expanding the known universe of RNA-binding proteins, methods for the discovery and characterization of protein-RNA interactions remain resource intensive and technically challenging. Here we introduce a UV-C crosslinking and immunoprecipitation platform, irCLIP, which provides an ultraefficient, fast, and nonisotopic method for the detection of protein-RNA interactions using far less material than standard protocols.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, N.I.H., Extramural

MeSH terms

  • Binding Sites
  • Cross-Linking Reagents / chemistry
  • DNA, Complementary / genetics
  • HeLa Cells
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Immunoprecipitation / methods*
  • Photochemical Processes
  • RNA-Binding Proteins / analysis*
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism
  • RNA-Binding Proteins / radiation effects
  • Sensitivity and Specificity
  • Transcriptome
  • Ultraviolet Rays*


  • Cross-Linking Reagents
  • DNA, Complementary
  • RNA-Binding Proteins