The effect of the glycolipoprotein extract (G-90) from earthworm Eisenia foetida on the wound healing process in alloxan-induced diabetic rats

Cell Biochem Funct. 2016 Jun;34(4):242-9. doi: 10.1002/cbf.3186. Epub 2016 Apr 25.


Diabetes is now regarded as a major public health problem. The number of patients is estimated to increase to over 439 million cases by 2030. One of the major health clinical problems in patients with diabetes patients is impaired wound healing. Diabetic foot ulcer is a major complication of diabetes mellitus in 12 to 25% of patients, which increases the risk of damage in the limbs or amputation. The earthworm Eisenia foetida glycolipoprotein (as known G-90) is a blend of macromolecules with some biological properties including mitogenicity, anticoagulation, fibrinolysis, bacteriostatic and antioxidatiaon. Given the biological properties of G-90, this study was conducted to investigate the effect of extract obtained from the homogenate of Eisenia foetida (G-90) on the wound healing process in alloxan-induced diabetic rats. The results of the present study revealed that treatment by using G-90 can speed up the wound healing process, which is exactly similar to the effect of D-panthenol treatment in rats. These findings also demonstrated that G-90 treatment decreases the risk of infection in the wound site compared with D-panthenol treatment. In addition, histological analysis indicated that a better extracellular matrix formation with increased fibroblast proliferation, neovascularization, collagen synthesis and early epithelial layer formation was observed in G-90 treated group. Therefore, the G-90 could be considered as a new wound healing agent introducing promising therapeutic approaches in both human and veterinary medicine. Copyright © 2016 John Wiley & Sons, Ltd.

Keywords: Eisenia foetida; G-90; diabetic rat; earthworm; foot ulcer; wound healing.

MeSH terms

  • Alloxan
  • Animals
  • Body Weight / drug effects
  • Diabetes Mellitus, Experimental / pathology*
  • Epithelium / drug effects
  • Epithelium / pathology
  • Female
  • Glycoproteins / pharmacology*
  • Lipoproteins / pharmacology*
  • Oligochaeta / chemistry*
  • Pantothenic Acid / analogs & derivatives
  • Pantothenic Acid / pharmacology
  • Rats, Wistar
  • Wound Healing / drug effects*


  • Glycoproteins
  • Lipoproteins
  • Pantothenic Acid
  • dexpanthenol
  • Alloxan