Influence of vitamin B6 status on aspects of lead poisoning in rats

Toxicol Lett. 1989 Apr;47(1):87-93. doi: 10.1016/0378-4274(89)90088-x.

Abstract

The effects of vitamin B6 status and lead (Pb) toxicity on aspects of glutathione (GSH) metabolism in rats were examined in a 2 x 2 factorial experiment. The administration of 2000 ppm Pb as Pb acetate.3H2O significantly (P less than 0.05) increased hepatic GSH levels in rats receiving B6-adequate (+B6) but decreased GSH in rats fed B6-deficient (-B6) diets. The addition of Pb to the +B6 diet also increased hepatic glutathione reductase (GSSG-R) activity. Plasma pyridoxal phosphate (PLP), cystathionine and valine levels were decreased by the -B6 diets independent of the presence of Pb. Plasma arginine, alanine, serine and proline levels were increased by Pb in both -B6 and +B6 groups. Glycine levels were increased in -B6 rats only in the absence of Pb while taurine was decreased by Pb only in +B6 rats. There were significant -B6 x Pb interactions for hepatic GSH, cysteine and GSSG-R as well as plasma valine, glycine and proline. These results suggest an influence of B6 status on Pb-induced changes in hepatic GSH, possibly through its role as a co-factor for enzymes involved in amino acid metabolism.

MeSH terms

  • Amino Acids / blood*
  • Animals
  • Coenzymes / administration & dosage
  • Cysteine / metabolism
  • Diet
  • Glutathione / metabolism
  • Glutathione Reductase / metabolism
  • Lead Poisoning / blood
  • Lead Poisoning / metabolism*
  • Liver / enzymology
  • Male
  • Pyridoxal Phosphate / blood
  • Pyridoxine / administration & dosage
  • Rats
  • Rats, Inbred Strains
  • Statistics as Topic
  • Vitamin B 6 Deficiency / metabolism*

Substances

  • Amino Acids
  • Coenzymes
  • Pyridoxal Phosphate
  • Glutathione Reductase
  • Glutathione
  • Cysteine
  • Pyridoxine