Occurrence of microcystins in water, bloom, sediment and fish from a public water supply

Sci Total Environ. 2016 Aug 15;562:860-868. doi: 10.1016/j.scitotenv.2016.04.027. Epub 2016 Apr 23.

Abstract

Microcystin (MC) accumulation was determined in the liver and muscle of two omnivorous fish species which are consumed and are economically important, and in a planktivorous-carnivorous fish from Lake Eğirdir, Turkey. Free extractable MCs in fish tissue samples were detected by enzyme-linked immunosorbent assay (ELISA) with confirmation by high performance liquid chromatography with photodiode array detection (HPLC-PDA). MC-LA and -YR, were detected in both liver and muscle, followed by MCs -LY, -LF, -RR and -LR respectively. The MC concentrations varied between 0.043 and 1.72μg/g dry weight in liver and muscle tissues. MCs were also determined in samples of water, sediment and a bloom sample of Microcystis aeruginosa from the lake by HPLC-PDA. MC-LY and -YR were most commonly identified in water samples, with total MC concentrations ranging from 2.9±0.05 to 13.5±2.3μg/L. Sediment analyses, showed that MC-YR was present in samples between 7.0 and 17.6μg/g dw, especially in October, November and December when no MC-YR was recorded in water, followed by MC-LW. The findings indicate that water and sediment contained MCs, and more importantly that fish were contaminated with MCs that may pose an MC-associated human health risk.

Keywords: ELISA; Fish tissue; HPLC analysis; Microcystins; Sediment; Water.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Environmental Monitoring*
  • Enzyme-Linked Immunosorbent Assay
  • Fishes / metabolism*
  • Fishes / microbiology
  • Harmful Algal Bloom*
  • Humans
  • Lakes / chemistry
  • Lakes / microbiology
  • Microcystins / analysis*
  • Microcystins / metabolism
  • Microcystis / growth & development*
  • Turkey
  • Water Microbiology*
  • Water Pollutants
  • Water Supply / statistics & numerical data

Substances

  • Microcystins
  • Water Pollutants
  • microcystin-LW
  • microcystin YR
  • microcystin LY
  • microcystin
  • cyanoginosin-LA