Protein-SIP in environmental studies

Curr Opin Biotechnol. 2016 Oct:41:26-33. doi: 10.1016/j.copbio.2016.04.010. Epub 2016 Apr 23.


Metaproteomics coupled to stable isotope probing (SIP) was established to detect metabolically active key players in microbial communities. Here, we discuss the current state of protein-based stable isotope probing (protein-SIP) and the perspectives of using different stable isotope atoms (i.e. 13C, 15N, 18O, 34/36S), multiple isotope labelling, the utilisation of substrates of major abundance and micro-pollutants [pesticides, herbicides and pharmaceuticals present in the environment at very low concentrations (ngμg/L)], and applications in complex model systems and in situ studies in the environment.

Publication types

  • Review

MeSH terms

  • Bacteria / metabolism*
  • Bacterial Proteins / metabolism*
  • Environmental Pollutants / analysis*
  • Isotope Labeling / methods*
  • Isotopes / analysis*


  • Bacterial Proteins
  • Environmental Pollutants
  • Isotopes