Validation of T2* in-line analysis for tissue iron quantification at 1.5 T

J Cardiovasc Magn Reson. 2016 Apr 27;18(1):23. doi: 10.1186/s12968-016-0243-4.


Background: There is a need for improved worldwide access to tissue iron quantification using T2* cardiovascular magnetic resonance (CMR). One route to facilitate this would be simple in-line T2* analysis widely available on MR scanners. We therefore compared our clinically validated and established T2* method at Royal Brompton Hospital (RBH T2*) against a novel work-in-progress (WIP) sequence with in-line T2* measurement from Siemens (WIP T2*).

Methods: Healthy volunteers (n = 22) and patients with iron overload (n = 78) were recruited (53 males, median age 34 years). A 1.5 T study (Magnetom Avanto, Siemens) was performed on all subjects. The same mid-ventricular short axis cardiac slice and transaxial slice through the liver were used to acquire both RBH T2* images and WIP T2* maps for each participant. Cardiac white blood (WB) and black blood (BB) sequences were acquired. Intraobserver, interobserver and interstudy reproducibility were measured on the same data from a subset of 20 participants.

Results: Liver T2* values ranged from 0.8 to 35.7 ms (median 5.1 ms) and cardiac T2* values from 6.0 to 52.3 ms (median 31 ms). The coefficient of variance (CoV) values for direct comparison of T2* values by RBH and WIP were 6.1-7.8 % across techniques. Accurate delineation of the septum was difficult on some WIP T2* maps due to artefacts. The inability to manually correct for noise by truncation of erroneous later echo times led to some overestimation of T2* using WIP T2* compared with the RBH T2*. Reproducibility CoV results for RBH T2* ranged from 1.5 to 5.7 % which were better than the reproducibility of WIP T2* values of 4.1-16.6 %.

Conclusions: Iron estimation using the T2* CMR sequence in combination with Siemens' in-line data processing is generally satisfactory and may help facilitate global access to tissue iron assessment. The current automated T2* map technique is less good for tissue iron assessment with noisy data at low T2* values.

Keywords: CMR; Heart; In-line processing; Iron overload; Liver; T2*.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Algorithms
  • Artifacts
  • Cardiomyopathies / diagnosis*
  • Cardiomyopathies / metabolism
  • Case-Control Studies
  • Female
  • Humans
  • Image Interpretation, Computer-Assisted
  • Iron / analysis*
  • Iron Overload / diagnosis*
  • Iron Overload / metabolism
  • Liver / chemistry
  • Liver / diagnostic imaging*
  • Liver Diseases / diagnosis*
  • Liver Diseases / metabolism
  • London
  • Magnetic Resonance Imaging, Cine*
  • Male
  • Middle Aged
  • Myocardium / chemistry*
  • Predictive Value of Tests
  • Reproducibility of Results
  • Young Adult


  • Iron