microRNA signatures in peripheral blood fail to detect acute cellular rejection after liver transplantation

Biomarkers. 2016 Dec;21(8):699-707. doi: 10.3109/1354750X.2016.1172113. Epub 2016 Apr 28.


Objective: We investigated whether microRNA signatures in whole blood samples are associated with acute cellular rejection (ACR) after liver transplantation.

Materials and methods: Blood samples were collected using Paxgene technology and analyzed by microarrays and quantitative real-time polymerase chain reaction (qRT-PCR).

Results: microRNA signatures failed to distinguish between 19 patients with ACR and 16 controls. Let-7b-5p and let-7c were upregulated in a subgroup of patients with ACR during the 6th and 7th postoperative days but failed in an independent validation of 20 patients.

Conclusion: microRNA signatures in whole blood processed by Paxgene technology are not suited for the detection of ACR after liver transplantation.

Keywords: Allograft; Paxgene; biomarker.