The effect of Aloe vera gel on viability of dental pulp stem cells

Dent Traumatol. 2016 Oct;32(5):390-6. doi: 10.1111/edt.12272. Epub 2016 Apr 29.


Background: Dental pulp stem cells (DPSCs) can play a prominent role in tissue regeneration. Aloe vera L. (Liliaceae) contains the polysaccharide of acemannan that was shown to be a trigger factor for cell proliferation, differentiation, mineralization, and dentin formation.

Aim: This study sought to determine the viability of DPSCs in Aloe vera in comparison with Hank's balanced salt solution (HBSS).

Materials and method: Twelve rabbits underwent anesthesia, and their incisor teeth were extracted; the pulp tissue was removed, chopped, treated with collagenase and plated in culture flasks. DPSCs from passage 3 were cultured in 24-well plates, and after 3 days, the culture media changed to 10, 25, 50, and 100% concentrations of Aloe vera at intervals of 45 and 90 min and 3 and 6 h. Distilled water was used as negative and HBSS as positive control for comparison. The cell morphology, viability, population doubling time (PDT), and growth kinetics were evaluated. RT-PCR was carried out for characterization and karyotyping for chromosomal stability.

Results: Aloe vera showed a significant higher viability than HBSS (74.74%). The 50% Aloe vera showed higher viability (97.73%) than other concentrations. PDT in 50% concentration was 35.1 h and for HBSS was 49.5 h. DPSCs were spindle shaped and were positive for CD73 and negative for CD34 and CD45. Karyotyping was normal.

Conclusions: Aloe vera as an inexpensive and available herb can improve survival of avulsed or broken teeth in emergency cases as a transfer media.

Keywords: Aloe vera; Hank's balanced salt solution; dental pulp stem cells; viability.

MeSH terms

  • Animals
  • Cell Survival
  • Cells, Cultured
  • Dental Pulp*
  • Plant Preparations / pharmacology*
  • Rabbits
  • Stem Cells*


  • Aloe vera gel
  • Plant Preparations