A Deep Insight Into the Sialome of Rhodnius Neglectus, a Vector of Chagas Disease

PLoS Negl Trop Dis. 2016 Apr 29;10(4):e0004581. doi: 10.1371/journal.pntd.0004581. eCollection 2016 Apr.

Abstract

Background: Triatomines are hematophagous insects that act as vectors of Chagas disease. Rhodnius neglectus is one of these kissing bugs found, contributing to the transmission of this American trypanosomiasis. The saliva of hematophagous arthropods contains bioactive molecules responsible for counteracting host haemostatic, inflammatory, and immune responses.

Methods/principal findings: Next generation sequencing and mass spectrometry-based protein identification were performed to investigate the content of triatomine R. neglectus saliva. We deposited 4,230 coding DNA sequences (CDS) in GenBank. A set of 636 CDS of proteins of putative secretory nature was extracted from the assembled reads, 73 of them confirmed by proteomic analysis. The sialome of R. neglectus was characterized and serine protease transcripts detected. The presence of ubiquitous protein families was revealed, including lipocalins, serine protease inhibitors, and antigen-5. Metalloproteases, disintegrins, and odorant binding protein families were less abundant.

Conclusions/significance: The data presented improve our understanding of hematophagous arthropod sialomes, and aid in understanding hematophagy and the complex interplay among vectors and their vertebrate hosts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Genomics
  • Insect Vectors*
  • Mass Spectrometry
  • Molecular Sequence Data
  • Peptide Hydrolases / analysis*
  • Peptide Hydrolases / genetics*
  • Proteomics
  • Rhodnius / physiology*
  • Saliva / chemistry*
  • Salivary Proteins and Peptides / analysis*
  • Salivary Proteins and Peptides / genetics*
  • Sequence Analysis, DNA

Substances

  • Salivary Proteins and Peptides
  • Peptide Hydrolases

Grant support

This work was supported by grants 476252/2012-1 (http://www.cnpq.br) from the Brazilian National Council for Scientific and Technological Development (CNPq), PROAP-2013 (http://www.capes.gov.br) from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), CT-Infra 2011 (http://www.finep.gov.br) from the Financiadora de Estudos e Projetos (Finep), and Pronex 476252/2012-5 (http://www.fap.df.gov.br) from the Fundação de Apoio à Pesquisa do Distrito Federal (FAPDF) to JMS; and Z01 AI000810-18 (http://www.niaid.nih.gov/Pages/default.aspx) to Vector-Borne Diseases: Biology of Vector Host Relationship, from the National Institute of Allergy and Infectious Diseases (NIAID) to JMCR. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.