Cerebrospinal fluid real-time quaking-induced conversion is a robust and reliable test for sporadic creutzfeldt-jakob disease: An international study

Ann Neurol. 2016 Jul;80(1):160-5. doi: 10.1002/ana.24679. Epub 2016 Jun 1.


Real-time quaking-induced conversion (RT-QuIC) has been proposed as a sensitive diagnostic test for sporadic Creutzfeldt-Jakob disease; however, before this assay can be introduced into clinical practice, its reliability and reproducibility need to be demonstrated. Two international ring trials were undertaken in which a set of 25 cerebrospinal fluid samples were analyzed by a total of 11 different centers using a range of recombinant prion protein substrates and instrumentation. The results show almost complete concordance between the centers and demonstrate that RT-QuIC is a suitably reliable and robust technique for clinical practice. Ann Neurol 2016;80:160-165.

Publication types

  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Case-Control Studies
  • Creutzfeldt-Jakob Syndrome / cerebrospinal fluid*
  • Creutzfeldt-Jakob Syndrome / diagnosis*
  • Female
  • Humans
  • Internationality
  • Male
  • Middle Aged
  • Prions / chemistry
  • Protein Aggregation, Pathological / cerebrospinal fluid*
  • Recombinant Proteins / chemistry
  • Reproducibility of Results
  • Sensitivity and Specificity


  • Prions
  • Recombinant Proteins

Supplementary concepts

  • Creutzfeldt-Jakob Disease, Sporadic