Isoorientin from Gypsophila elegans induces apoptosis in liver cancer cells via mitochondrial-mediated pathway

J Ethnopharmacol. 2016 Jul 1;187:187-94. doi: 10.1016/j.jep.2016.04.050. Epub 2016 Apr 27.

Abstract

Ethnopharmacological relevance: Gypsophila elegans has been used as a traditional herbal medicine for treating immune disorders and chronic liver diseases in China. The aim of this study is to isolate an active ingredient from this herb and investigate its anti-tumor activity.

Materials and methods: An active ingredient was isolated from the ethanol extract using bioassay-guided screening. And its anti-tumor activity was analyzed by testing the cytotoxicity, lactate dehydrogenase (LDH) release, clonogenecity and migration in HepG2 cells. To investigate its potential mechanism, cell apoptosis, cell cycle arrest, reactive oxygen species (ROS), cytochrome c, mitochondria membrane potential (MMP) and caspase level were determined in liver cancer cell line HepG2.

Results: A flavonoid glycoside, i.e., G. elegans isoorientin (GEI), was isolated from this herb and identified as Isoorientin-2″-O-α-l-arabinopyranosyl. Our results showed that GEI significantly inhibited the viability and proliferation of HepG2 cells in a dose- and time-dependent manner, and its cytotoxic effect was also confirmed by the elevated level of LDH. GEI treatment could markedly inhibit the clonogenicity and migration of HepG2 cells. Moreover, GEI induced remarkable apoptotic death of HepG2 cells through cell cycle arrest at G1 phase via the regulation of cell cycle-related genes, such as cyclin D, cyclin E and CDK2. Further study showed that GEI treatment significantly elevated ROS formation, followed by attenuation of MMP via up-regulation of Bax and down-regulation of Bcl-2, accompanied by cytochrome c release to the cytosol. In addition, GEI treatment resulted in a significant dose-dependent increase in caspase-3 and -9 proteolytic activities.

Conclusion: The present study demonstrates that the ability of GEI to induce apoptosis against HepG2 cells mediated by mitochondrial-mediated pathway.

Keywords: Cytotoxicity; Gypsophila elegans; Isoorientin; Mitochondrial-mediated pathway; Reactive oxygen species.

MeSH terms

  • Antineoplastic Agents / pharmacology*
  • Apoptosis / drug effects
  • Caryophyllaceae*
  • Caspases / metabolism
  • Cell Cycle / drug effects
  • Cell Movement / drug effects
  • Cell Survival / drug effects
  • Cyclin D / genetics
  • Cyclin E / genetics
  • Cyclin-Dependent Kinase 2 / genetics
  • Hep G2 Cells
  • Humans
  • L-Lactate Dehydrogenase / metabolism
  • Luteolin / pharmacology*
  • Membrane Potential, Mitochondrial / drug effects
  • Mitochondria / drug effects*
  • Mitochondria / physiology
  • Proto-Oncogene Proteins c-bcl-2 / genetics
  • Reactive Oxygen Species / metabolism
  • bcl-2-Associated X Protein / genetics

Substances

  • Antineoplastic Agents
  • BAX protein, human
  • Cyclin D
  • Cyclin E
  • Proto-Oncogene Proteins c-bcl-2
  • Reactive Oxygen Species
  • bcl-2-Associated X Protein
  • homoorientin
  • L-Lactate Dehydrogenase
  • CDK2 protein, human
  • Cyclin-Dependent Kinase 2
  • Caspases
  • Luteolin