Clinical and Prognostic Significance of ANCCA in Squamous Cell Lung Carcinoma Patients

Arch Med Res. 2016 Feb;47(2):89-95. doi: 10.1016/j.arcmed.2016.04.001. Epub 2016 Apr 27.


Background and aims: AAA+ nuclear coregulatory cancer associated (ANCCA) has been demonstrated as playing important roles in diverse biological processes including tumorigenesis. However, the clinical and prognostic significance of ANCCA in squamous cell lung carcinoma (SCLC) patients is still unknown. The aim of this study is to identify the role of ANCCA in SCLC patients.

Methods: ANCCA mRNA and protein expressions were detected in SCLC tissues and cell lines by real-time PCR and Western blot. We examined the ANCCA protein expression in 152 SCLC samples by immunohistochemistry and analyzed the association between the expression of ANCCA protein and clinicopathological characteristics of SCLC patients.

Results: ANCCA mRNA and protein expression are increased in SCLC tissues and cell lines. Moreover, ANCCA protein overexpression was associated with differentiated degree, clinical stage, lymph node metastasis, and distant metastasis. In uni- and multivariate analyses, ANCCA protein overexpression was an independent poor prognostic factor for SCLC patients.

Conclusions: ANCCA act as a potential biomarker for therapeutic strategy and prognostic prediction for SCLC.

Keywords: ANCCA; Biomarker; Lung squamous cell carcinoma; Prognosis.

MeSH terms

  • ATPases Associated with Diverse Cellular Activities
  • Adenosine Triphosphatases / genetics
  • Adenosine Triphosphatases / metabolism*
  • Carcinoma, Squamous Cell / diagnosis
  • Carcinoma, Squamous Cell / pathology*
  • Cell Line, Tumor
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Female
  • Humans
  • Lung Neoplasms / diagnosis
  • Lung Neoplasms / pathology*
  • Lymphatic Metastasis
  • Male
  • Middle Aged
  • Neoplasm Metastasis
  • Prognosis
  • RNA, Messenger / metabolism


  • DNA-Binding Proteins
  • RNA, Messenger
  • Adenosine Triphosphatases
  • ATAD2 protein, human
  • ATPases Associated with Diverse Cellular Activities