Diversity and Tropism of HIV-1 Rebound Virus Populations in Plasma Level After Treatment Discontinuation

J Infect Dis. 2016 Aug 1;214(3):403-7. doi: 10.1093/infdis/jiw172. Epub 2016 Apr 30.

Abstract

Human immunodeficiency virus-infected people discontinuing therapy experience a rebound in the virus level (hereafter, "rebound virus") from a persistent reservoir. We examined 10 samples from patients in AIDS Clinical Trials Group study A5068 with rebound virus, using single-genome amplification and Primer ID deep sequencing, to assess env genetic diversity of the virus population. Most rebound-virus populations showed significant diversity. All env examined required high levels of CD4 for entry, consistent with selection of replication in CD4(+) T cells. These results indicate that most people discontinuing therapy release a diverse population of virus and that this released virus has entry features of virus selected for replication in CD4(+) T cells, rather than in myeloid cells.

Keywords: HIV-1; genetic diversity; rebound virus; reservoir; tropism.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • HIV Infections / virology*
  • HIV-1 / classification
  • HIV-1 / genetics
  • HIV-1 / isolation & purification*
  • HIV-1 / physiology*
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Plasma / virology*
  • Sequence Analysis, DNA
  • Viral Load*
  • Viral Tropism*
  • Withholding Treatment*
  • env Gene Products, Human Immunodeficiency Virus / genetics

Substances

  • env Gene Products, Human Immunodeficiency Virus