miR-195 inhibits tumor growth and angiogenesis through modulating IRS1 in breast cancer

Biomed Pharmacother. 2016 May:80:95-101. doi: 10.1016/j.biopha.2016.03.007. Epub 2016 Mar 17.


Angiogenesis has been found as an attractive target for drug therapy as it is necessary for tumor growth. Accumulating evidences show that microRNAs (miRNAs), which are a group of highly conserved, single-stranded, short non-coding RNAs, play important roles through directly targeting angiogenic factors and protein kinases. The purpose of this study is to investigate the role of miR-195 in breast cancer development and angiogenesis through targeting IRS1. We show that miR-195 is inversely related with Insulin receptor substrate 1 (IRS1) in both breast cancer cells and breast cancer tissues. Induction of miR-195 could suppress IRS1 protein expression through binding to its 3'UTR regions either by transfection with miR-195 oligo or by infection with lentivirus encoding miR-195 gene. Moreover, re-expression of IRS1 reverses miR-195-mediated repression of tumor cell growth and miR-195 inhibits tumor angiogenesis through suppressing IRS1-VEGF axis. These data suggest that miR-195 mimics are potential therapeutic agents for breast cancer diagnose.

Keywords: Angiogenesis; Breast cancer; Insulin receptor substrate 1; MiR-195.

MeSH terms

  • Animals
  • Base Sequence
  • Breast Neoplasms / blood supply*
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / pathology
  • Cell Line, Tumor
  • Cell Proliferation
  • Chickens
  • Down-Regulation / genetics
  • Female
  • Gene Expression Regulation, Neoplastic
  • Gene Knockdown Techniques
  • Humans
  • Insulin Receptor Substrate Proteins / genetics*
  • Insulin Receptor Substrate Proteins / metabolism
  • MicroRNAs / metabolism*
  • Neovascularization, Pathologic / genetics*
  • Reproducibility of Results


  • Insulin Receptor Substrate Proteins
  • MIRN195 microRNA, human
  • MicroRNAs