Prototype of Multiplex Bead Assay for Quantification of Three Serum Biomarkers for In Vitro Diagnosis of Endometriosis

J Cell Physiol. 2016 Dec;231(12):2622-7. doi: 10.1002/jcp.25410. Epub 2016 May 16.


Endometriosis is a very common disease, affecting 10% of women in the reproductive age. To date, a significant delay between onset of the symptoms and definitive diagnosis is caused by the lack of a reliable non-invasive diagnostic test. Recently, the potential value as diagnostic markers for endometriosis of three proteins (Zn-alpha2-glycoprotein, serum albumin, and complement C3 precursor), has been showed. In this article, we have defined the experimental conditions for the development of a multiplex bead array assay for rapid and simultaneous quantification of these three biomarkers in the serum of patients with endometriosis. Finally, pivotal experiments on a small cohort of patients have confirmed the diagnostic value of this assay. J. Cell. Physiol. 231: 2622-2627, 2016. © 2016 Wiley Periodicals, Inc.

MeSH terms

  • Adipokines
  • Adult
  • Biological Assay / methods*
  • Biomarkers / blood*
  • Calibration
  • Carrier Proteins / blood
  • Case-Control Studies
  • Complement C3 / metabolism
  • Endometriosis / blood*
  • Endometriosis / diagnosis*
  • Female
  • Glycoproteins / blood
  • Humans
  • Protein Binding
  • Serum Albumin / metabolism


  • AZGP1 protein, human
  • Adipokines
  • Biomarkers
  • Carrier Proteins
  • Complement C3
  • Glycoproteins
  • Serum Albumin