Secretory cargo sorting by Ca2+-dependent Cab45 oligomerization at the trans-Golgi network

J Cell Biol. 2016 May 9;213(3):305-14. doi: 10.1083/jcb.201601089. Epub 2016 May 2.


Sorting and export of transmembrane cargoes and lysosomal hydrolases at the trans-Golgi network (TGN) are well understood. However, elucidation of the mechanism by which secretory cargoes are segregated for their release into the extracellular space remains a challenge. We have previously demonstrated that, in a reaction that requires Ca(2+), the soluble TGN-resident protein Cab45 is necessary for the sorting of secretory cargoes at the TGN. Here, we report that Cab45 reversibly assembles into oligomers in the presence of Ca(2+) These Cab45 oligomers specifically bind secretory proteins, such as COMP and LyzC, in a Ca(2+)-dependent manner in vitro. In intact cells, mutation of the Ca(2+)-binding sites in Cab45 impairs oligomerization, as well as COMP and LyzC sorting. Superresolution microscopy revealed that Cab45 colocalizes with secretory proteins and the TGN Ca(2+) pump (SPCA1) in specific TGN microdomains. These findings reveal that Ca(2+)-dependent changes in Cab45 mediate sorting of specific cargo molecules at the TGN.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biological Transport
  • Calcium / metabolism
  • Calcium Signaling*
  • Calcium-Binding Proteins / metabolism
  • Calcium-Binding Proteins / physiology*
  • Glycoproteins / metabolism
  • Glycoproteins / physiology*
  • HeLa Cells
  • Humans
  • Models, Molecular
  • Proteins / metabolism
  • Secretory Pathway
  • trans-Golgi Network / metabolism*


  • Calcium-Binding Proteins
  • Glycoproteins
  • Proteins
  • SDF4 protein, human
  • Calcium