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. 2016 Nov 1;95(11):2624-2632.
doi: 10.3382/ps/pew160. Epub 2016 May 3.

Therapeutic Effect of Flax-Based Diets on Fatty Liver in Aged Laying Hens

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Free PMC article

Therapeutic Effect of Flax-Based Diets on Fatty Liver in Aged Laying Hens

J E Davis et al. Poult Sci. .
Free PMC article

Abstract

This study examined the ability of flax-based ingredients to attenuate nonalcoholic fatty liver disease ( NAFLD: ) in aged laying hens-a novel and more physiologically relevant model of human disease. Our results showed only hens supplemented with whole flaxseed ( WFX: ) reduced steatosis and hepatocellular ballooning. Serum AST was also reduced in hens provided WFX and defatted flaxseed meal ( DFM: ). Hepatic ω-3 PUFA enrichment was improved with supplementation of WFX, DFM, and flaxseed oil ( FXO: ). However, this effect was more evident in the WFX group. In contrast, transcript abundance of genes linked to NAFLD were predominantly modified with FXO supplementation. Taken together, our data indicate a potential synergistic relationship between the fatty acid and lignan content in flaxseed which attenuated the progression of NAFLD in aged laying hens. Although more research is necessary, these findings demonstrate the potential use of whole flaxseed for the treatment and prevention of NAFLD in humans.

Keywords: Flaxseed; hepatic steatosis; laying hen; lignan; omega-3 fatty acid.

Figures

Figure 1.
Figure 1.
Liver histology by hematoxylin and eosin (H&E) staining. Macrovascular steatosis (S1), microvascular steatosis (S2), hepatocyte ballooning (B), and hepatic inflammation were evaluated in liver tissue of laying hens fed experimental diets for 24 wk (as described in Materials and Methods). Representative images of H&E stained liver tissue are presented at 40× (magnification bar = 200 μm).
Figure 2.
Figure 2.
Liver histology by Masson's trichrome staining. Histological changes in liver tissue observed by Masson's trichrome staining in laying hens on experimental diets for 24 wk (as described in Materials and Methods). Representative images of trichrome stained liver tissue are presented at 10× (magnification bar = 200 μm).

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