Production of Heteromeric Transmembrane Receptors with Defined Subunit Stoichiometry

Structure. 2016 May 3;24(5):653-655. doi: 10.1016/j.str.2016.04.004.


Signal transduction across cell membranes often requires assembly of heteromeric receptors with defined stoichiometry. In this issue of Structure, Morales-Perez et al. (2016) present elegant methods for the expression of heteromeric nicotinic acetylcholine receptors with a defined α4β2 stoichiometry involving controlled baculovirus-mediated transduction and subunit counting by measurement of two fluorescent signals.

Publication types

  • Comment

MeSH terms

  • Receptors, Nicotinic / chemistry*


  • Receptors, Nicotinic