Sequence analysis of cloned dengue virus type 2 genome (New Guinea-C strain)

Gene. 1989 Feb 20;75(2):197-211. doi: 10.1016/0378-1119(89)90266-7.


Sequences totalling 5472 nucleotides (nt) from four complementary DNA (cDNA) clones of the dengue virus type 2 (DEN-2) RNA (New Guinea strain, NGS-C) have been reported previously [Yaegashi et al., Gene 46 (1986) 257-267; Putnak et al., Virology 163 (1988) 93-103]. This report describes the complete nucleotide sequence, with the exception of about 7 nt at the 5'-noncoding region, of this RNA genome derived from several cDNA clones. It is 10,723 nt in length and contains a single long open reading frame of 10,173 nt, encoding a polyprotein of 3391 amino acids. The genomic organization is similar to that of other flaviviruses that have recently been reported. Among the three DEN-2 strains - the Jamaica genotype (DEN-2JAM), the DEN-2NGS-C, and the S1 candidate vaccine strain derived from Puerto Rico (PR)-159 isolate (DEN-2S1) - which have been sequenced to date, the amino acid sequences of the polyproteins bear 94%-99% similarity. When the amino acid sequences of DEN-2NGS-C are compared with those of the other two strains, the variations are greater in the DEN-2S1 than in the DEN-2JAM. When DEN-2 and DEN-4 are compared, the overall amino acid identities range from 30% to 80% in both the structural and nonstructural proteins; whereas between DEN-2 and DEN-1, they range from 68% to 79% in the region encoding the structural proteins and the nonstructural protein NS1.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Biological Evolution
  • Cloning, Molecular
  • Cysteine / genetics
  • DNA
  • Dengue Virus / genetics*
  • Dengue Virus / immunology
  • Genes, Viral*
  • Genetic Variation*
  • Glycosylation
  • Molecular Sequence Data
  • New Guinea
  • Protein Processing, Post-Translational
  • RNA, Viral / genetics
  • Serotyping
  • Species Specificity
  • Viral Proteins / genetics*


  • RNA, Viral
  • Viral Proteins
  • DNA
  • Cysteine

Associated data

  • GENBANK/M19727
  • GENBANK/M29095