Backbone and side-chain (1)H, (13)C, and (15)N chemical shift assignments for the apo-form of the lytic polysaccharide monooxygenase NcLPMO9C

Gaston Courtade; Reinhard Wimmer; Maria Dimarogona; Mats Sandgren; Vincent G H Eijsink; Finn L Aachmann

doi:10.1007/s12104-016-9683-x

Backbone and side-chain (1)H, (13)C, and (15)N chemical shift assignments for the apo-form of the lytic polysaccharide monooxygenase NcLPMO9C

Biomol NMR Assign. 2016 Oct;10(2):277-80. doi: 10.1007/s12104-016-9683-x. Epub 2016 May 4.

Authors

Gaston Courtade¹, Reinhard Wimmer², Maria Dimarogona³, Mats Sandgren³, Vincent G H Eijsink⁴, Finn L Aachmann⁵

Affiliations

¹ NOBIPOL, Department of Biotechnology, Norwegian University of Science and Technology (NTNU), Sem Sælands vei 6/8, 7491, Trondheim, Norway.
² Department of Chemistry and Bioscience, Aalborg University, Frederik Bajers vej 7H, 9220, Aalborg Ø, Denmark.
³ Department of Chemistry and Biotechnology, Swedish University of Agricultural Sciences, 750 07, Uppsala, Sweden.
⁴ Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, 1432, Ås, Norway.
⁵ NOBIPOL, Department of Biotechnology, Norwegian University of Science and Technology (NTNU), Sem Sælands vei 6/8, 7491, Trondheim, Norway. finn.l.aachmann@ntnu.no.

PMID: 27147444
DOI: 10.1007/s12104-016-9683-x

Abstract

The apo-form of the 23.3 kDa catalytic domain of the AA9 family lytic polysaccharide monooxygenase NcLPMO9C from Neurospora crassa has been isotopically labeled and recombinantly expressed in Pichia pastoris. In this paper, we report the (1)H, (13)C, and (15)N chemical shift assignments of this LPMO.

Keywords: AA9; Cellulose; LPMO; Lytic polysaccharide monooxygenase; Xyloglucan.

MeSH terms

Apoenzymes / chemistry*
Apoenzymes / metabolism*
Mixed Function Oxygenases / chemistry*
Mixed Function Oxygenases / metabolism*
Neurospora crassa / enzymology*
Nuclear Magnetic Resonance, Biomolecular*
Polysaccharides / metabolism*

Substances

Apoenzymes
Polysaccharides
Mixed Function Oxygenases