Generation of Human Liver Chimeric Mice for the Study of Human Hepatotropic Pathogens

Methods Mol Biol. 2016;1438:79-101. doi: 10.1007/978-1-4939-3661-8_5.


Human liver chimeric mice have become valuable tools for the study of human hepatotropic pathogens and for the investigation of metabolism and pharmacokinetics of novel drugs. The evolution of the underlying mouse models has been rapid in the past years. The diverse fields of applications of those model systems and their technical challenges will be discussed in this chapter.

Keywords: DMPK; Hepatitis B virus; Hepatitis C virus; Hepatitis delta virus; Human liver chimeric mice; Humanized mice; Malaria; Viral hepatitis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cryopreservation
  • Disease Models, Animal
  • Hepatocytes / cytology
  • Hepatocytes / transplantation*
  • Humans
  • Liver / cytology*
  • Liver / growth & development
  • Liver Regeneration
  • Mice
  • Transplantation Chimera / growth & development*
  • Transplantation, Heterologous