CD55 is a key complement regulatory protein that counteracts complement-mediated inactivation of Newcastle Disease Virus

J Gen Virol. 2016 Aug;97(8):1765-1770. doi: 10.1099/jgv.0.000498. Epub 2016 May 6.

Abstract

Newcastle disease virus (NDV) is being developed as an oncolytic virus for virotherapy. In this study we analysed the regulation of complement-mediated inactivation of a recombinant NDV in different host cells. NDV grown in human cells was less sensitive to complement-mediated virus inactivation than NDV grown in embryonated chicken eggs. Additionally, NDV produced from HeLa-S3 cells is more resistant to complement than NDV from 293F cells, which correlated with higher expression and incorporation of complement regulatory proteins (CD46, CD55 and CD59) into virions from HeLa-S3 cells. Further analysis of the recombinant NDVs individually expressing the three CD molecules showed that CD55 is the most potent in counteracting complement-mediated virus inactivation. The results provide important information on selecting NDV manufacture substrate to mitigate complement-mediated virus inactivation.

MeSH terms

  • Animals
  • CD55 Antigens / metabolism*
  • CD59 Antigens / metabolism
  • Cell Line
  • Chickens
  • Complement Inactivator Proteins / metabolism*
  • Complement System Proteins / metabolism*
  • Host-Pathogen Interactions*
  • Humans
  • Immunologic Factors / metabolism*
  • Membrane Cofactor Protein / metabolism
  • Newcastle disease virus / immunology*
  • Newcastle disease virus / physiology*

Substances

  • CD55 Antigens
  • CD59 Antigens
  • Complement Inactivator Proteins
  • Immunologic Factors
  • Membrane Cofactor Protein
  • Complement System Proteins