Abstract
To investigate the feasibility and efficacy of a virus-like particle (VLP) vaccine composed of the conserved antigenic epitopes of respiratory syncytial virus (RSV), the chimeric RSV VLPs HBcΔ-tG and HBcΔ-tG/M282-90 were generated based on the truncated hepatitis B virus core protein (HBcΔ). HBcΔ-tG consisted of HBcΔ, the conserved region (aa 144-204) of the RSV G protein. HBcΔ-tG was combined with a single peptide (aa 82-90) of the M2 protein to generate HBcΔ-tG/M282-90. Immunization of mice with the HBcΔ-tG or HBcΔ-tG/M282-90 VLPs elicited RSV-specific IgG and neutralizing antibody production and conferred protection against RSV infection. Compared with HBcΔ-tG, HBcΔ-tG/M282-90 induced decreased Th2 cytokine production (IL-4 and IL-5), increased Th1 cytokine response (IFN-γ, TNF-α, and IL-2), and increased ratios of IgG2a/IgG1 antibodies, thereby relieving pulmonary pathology upon subsequent RSV infection. Our results demonstrated that chimeric HBcΔ-tG/M282-90 VLPs represented an effective RSV subunit vaccine candidate.
Keywords:
Hepatitis B virus core protein (HBc); Lung pathology; RSV attachment glyco (G) protein; Respiratory syncytial virus (RSV); Subunit vaccine; Virus like particle (VLP).
Copyright © 2016 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Neutralizing / blood
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Antibodies, Viral / blood
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Cell Line
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Cytokines / biosynthesis
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Cytokines / immunology
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Epitopes / immunology
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Hepatitis B Core Antigens / genetics
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Hepatitis B Core Antigens / immunology
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Immunoglobulin G / blood
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Lung / immunology
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Lung / pathology
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Lung / virology
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Mice
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Mice, Inbred BALB C
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Respiratory Syncytial Virus Infections / immunology
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Respiratory Syncytial Virus Infections / prevention & control*
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Respiratory Syncytial Virus Vaccines / administration & dosage
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Respiratory Syncytial Virus Vaccines / genetics
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Respiratory Syncytial Virus Vaccines / immunology*
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Respiratory Syncytial Virus, Human / genetics
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Respiratory Syncytial Virus, Human / immunology*
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Vaccines, Subunit / administration & dosage
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Vaccines, Subunit / genetics
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Vaccines, Subunit / immunology
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Vaccines, Virus-Like Particle / genetics
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Vaccines, Virus-Like Particle / immunology*
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Viral Fusion Proteins / genetics
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Viral Fusion Proteins / immunology*
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Viral Matrix Proteins / genetics
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Viral Matrix Proteins / immunology*
Substances
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Antibodies, Neutralizing
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Antibodies, Viral
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Cytokines
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Epitopes
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G glycoprotein, Respiratory syncytial virus
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Hepatitis B Core Antigens
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Immunoglobulin G
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Respiratory Syncytial Virus Vaccines
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Vaccines, Subunit
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Vaccines, Virus-Like Particle
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Viral Fusion Proteins
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Viral Matrix Proteins