Structures of the Zika Virus Envelope Protein and Its Complex with a Flavivirus Broadly Protective Antibody

Cell Host Microbe. 2016 May 11;19(5):696-704. doi: 10.1016/j.chom.2016.04.013. Epub 2016 May 2.


Zika virus (ZIKV), a mosquito-borne flavivirus, is a current global public health concern. The flavivirus envelope (E) glycoprotein is responsible for virus entry and represents a major target of neutralizing antibodies for other flaviviruses. Here, we report the structures of ZIKV E protein at 2.0 Å and in complex with a flavivirus broadly neutralizing murine antibody 2A10G6 at 3.0 Å. ZIKV-E resembles all the known flavivirus E structures but contains a unique, positively charged patch adjacent to the fusion loop region of the juxtaposed monomer, which may influence host attachment. The ZIKV-E-2A10G6 complex structure reveals antibody recognition of a highly conserved fusion loop. 2A10G6 binds to ZIKV-E with high affinity in vitro and neutralizes currently circulating ZIKV strains in vitro and in mice. The E protein fusion loop epitope represents a potential candidate for therapeutic antibodies against ZIKV.

MeSH terms

  • Animals
  • Antibodies, Neutralizing / immunology
  • Antibodies, Viral / immunology
  • Cell Line
  • Crystallization
  • Disease Models, Animal
  • Epitopes / immunology
  • Flavivirus / chemistry
  • Flavivirus / immunology*
  • Flavivirus Infections / virology
  • Male
  • Mice
  • Models, Molecular
  • Protein Conformation
  • Viral Envelope Proteins / chemistry
  • Viral Envelope Proteins / immunology*
  • Virus Internalization
  • Zika Virus / chemistry
  • Zika Virus / immunology*
  • Zika Virus Infection / virology


  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Epitopes
  • Viral Envelope Proteins