Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Jul 15;5(7):582-8.
doi: 10.1021/acssynbio.6b00056. Epub 2016 May 9.

Multiplexed Targeted Genome Engineering Using a Universal Nuclease-Assisted Vector Integration System

Affiliations

Multiplexed Targeted Genome Engineering Using a Universal Nuclease-Assisted Vector Integration System

Alexander Brown et al. ACS Synth Biol. .

Abstract

Engineered nucleases are capable of efficiently modifying complex genomes through introduction of targeted double-strand breaks. However, mammalian genome engineering remains limited by low efficiency of heterologous DNA integration at target sites, which is typically performed through homologous recombination, a complex, ineffective and costly process. In this study, we developed a multiplexable and universal nuclease-assisted vector integration system for rapid generation of gene knock outs using selection that does not require customized targeting vectors, thereby minimizing the cost and time frame needed for gene editing. Importantly, this system is capable of remodeling native mammalian genomes through integration of DNA, up to 50 kb, enabling rapid generation and screening of multigene knockouts from a single transfection. These results support that nuclease assisted vector integration is a robust tool for genome-scale gene editing that will facilitate diverse applications in synthetic biology and gene therapy.

Keywords: CRISPR; DNA recombination; TALEN; gene editing; genome engineering; synthetic biology; targeted genome integration.

Similar articles

See all similar articles

Cited by 8 articles

See all "Cited by" articles

Substances

LinkOut - more resources

Feedback