Complete sequence of human mitochondrial DNA obtained by combining multiple displacement amplification and next-generation sequencing on a single oocyte

Mitochondrial DNA A DNA Mapp Seq Anal. 2017 Mar;28(2):180-181. doi: 10.3109/19401736.2015.1115499. Epub 2016 Feb 24.

Abstract

Mitochondrial DNA (mtDNA) plays a key role in the development of a competent oocyte. In this study, the complete mtDNA sequence obtained for the first time by multiple displacement amplification approach in combination with next-generation sequencing from a single human oocyte is reported (GenBank accession no. KT364276). The analysis of oocyte mitochondrial mutations could provide a better understanding of the genetic variants correlated with the oocyte quality.

Keywords: Heteroplasmic mutations; human oocyte; mitochondrial DNA; next-generation sequencing.

MeSH terms

  • DNA Mutational Analysis / methods*
  • DNA, Mitochondrial
  • Genes, Mitochondrial*
  • Genome, Mitochondrial*
  • Genomics / methods
  • High-Throughput Nucleotide Sequencing / methods*
  • Humans
  • Mutation
  • Oocytes*

Substances

  • DNA, Mitochondrial