Histopathological Evaluation of the Effects of CAPE in Experimental Spinal Cord Injury

Turk Neurosurg. 2016;26(3):437-44. doi: 10.5137/1019-5149.JTN.11255-14.0.


Aim: < /B > Spinal cord injuries negatively affect the individuals and the life quality of their families due to neurological deficits caused by trauma. The prevalence of spinal cord injury is 15-45/1 million in the world. Caffeic acid phenethyl ester (CAPE) is the most active component of propolis and has neuroprotective, anti-oxidant and anti-apoptotic effects. Our aim was to determine the effects of CAPE on the prevention of secondary injury and to compare with methylprednisolone.

Material and methods: Forty rats were divided into 4 groups. The control group did not undergo surgery (Group I), only trauma group (Group II), trauma+CAPE treatment group (Group III), and trauma+methylprednisolone treatment group (Group IV). Histopathological assessment was performed with two staining methods as hematoxylin-eosin (HE) and terminal deoxynucleotidyl Transferase Biotin - dUTP Nick End Labeling (TUNEL). The groups were statistically compared.

Results: The apoptotic cells decreased in treatment groups compared with the trauma. CAPE has more anti-apoptotic effect than methylprednisolone. The histological difference between the Group II, and Groups III and IV was statistically significant.

Conclusion: CAPE has a positive effect on spinal cord injuries by preventing apoptosis.

Publication types

  • Comparative Study

MeSH terms

  • Acetaminophen / therapeutic use
  • Analgesics, Non-Narcotic / therapeutic use
  • Animals
  • Apoptosis / drug effects
  • Caffeic Acids / therapeutic use*
  • Female
  • In Situ Nick-End Labeling
  • Male
  • Methylprednisolone / therapeutic use
  • Neuroprotective Agents / therapeutic use*
  • Phenylethyl Alcohol / analogs & derivatives*
  • Phenylethyl Alcohol / therapeutic use
  • Rats
  • Rats, Sprague-Dawley
  • Spinal Cord Injuries / drug therapy*


  • Analgesics, Non-Narcotic
  • Caffeic Acids
  • Neuroprotective Agents
  • Acetaminophen
  • caffeic acid phenethyl ester
  • Phenylethyl Alcohol
  • Methylprednisolone