BODIPY-labeled Fluorescent Aptamer Sensors for Turn-on Sensing of Interferon-gamma and Adenine Compounds on Cells

Akira Tsuchiya; Siti N Hashim; Shoko Ise; Takafumi Furuhata; Kiyohiko Kawai; Rie Wakabayashi; Masahiro Goto; Noriho Kamiya; Shinsuke Sando

doi:10.2116/analsci.32.543

BODIPY-labeled Fluorescent Aptamer Sensors for Turn-on Sensing of Interferon-gamma and Adenine Compounds on Cells

Anal Sci. 2016;32(5):543-7. doi: 10.2116/analsci.32.543.

Authors

Akira Tsuchiya¹, Siti N Hashim, Shoko Ise, Takafumi Furuhata, Kiyohiko Kawai, Rie Wakabayashi, Masahiro Goto, Noriho Kamiya, Shinsuke Sando

Affiliation

¹ Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo.

PMID: 27169654
DOI: 10.2116/analsci.32.543

Abstract

An on-cell aptamer sensor has the potential to reveal cell-cell communications by signalling molecules. We attempted to design new fluorescent aptamer sensors for the sensing of IFN-γ and adenine compounds on cells. BODIPY-labeled external quencher-free aptamer sensors have allowed a turn-on detection of the target molecule with improved off/on efficiency.

MeSH terms

Adenine / analysis*
Adenine / chemistry
Aptamers, Nucleotide / chemistry*
Biosensing Techniques / methods*
Boron Compounds / chemistry*
Cells, Cultured
Flow Cytometry / methods*
Fluorescent Dyes / chemistry*
Humans
Interferon-gamma / analysis*
Interferon-gamma / chemistry
Molecular Structure

Substances

4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
Aptamers, Nucleotide
Boron Compounds
Fluorescent Dyes
Interferon-gamma
Adenine