Effects of carvedilol on an ischemia/reperfusion model: Biochemical, histopathological and immunohistochemical evaluation

J Obstet Gynaecol Res. 2016 Sep;42(9):1132-40. doi: 10.1111/jog.13028. Epub 2016 May 12.


Aim: The aim of this study was to investigate the effects of carvedilol (CVD) on experimentally induced ovarian ischemia/reperfusion (I/R) injury in rats.

Methods: An ovarian I/R model was applied to rats, classified into three groups: 1 (n = 7), sham operated (control); 2 (n = 7), 3 h ischemia + 3 h reperfusion (I/R); 3 (n = 7), 3 h ischemia + CVD + 3 h reperfusion (I/R + CVD). Malondialdehyde (MDA) levels and glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities in ovarian tissues and serum were measured. Tissue damage was examined histopathologically; Bax and caspase-3 expression was determined immunhistochemically. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was performed to show apoptotic cell death.

Results: MDA levels in ovarian tissues were significantly increased in the I/R group compared with the control. CVD administration significantly decreased tissue MDA levels in the I/R + CVD in comparison with the I/R group. GSH-Px activities in serum were higher in the I/R + CVD than in the I/R group. SOD activities in tissue and serum were significantly decreased in the I/R compared with the control group. Histological examination showed a significant improvement in ovarian morphology in the I/R + CVD compared with the I/R group. Bax and caspase-3 protein was more strongly expressed in the I/R group compared with the control and I/R + CVD groups. Apoptotic index detected by TUNEL assay was significantly increased in the I/R and decreased in the I/R + CVD group.

Conclusion: Our results suggest that CVD reduces the deleterious effects of oxidative damage on ovaries in a rat I/R model.

Keywords: Carvediol; ischemia/reperfusion injury; ovarian torsion; rat model.

MeSH terms

  • Adrenergic alpha-1 Receptor Antagonists / administration & dosage*
  • Animals
  • Apoptosis / drug effects
  • Carbazoles / administration & dosage*
  • Carvedilol
  • Caspase 3 / metabolism
  • Disease Models, Animal
  • Female
  • Glutathione Peroxidase / metabolism
  • Immunohistochemistry
  • Malondialdehyde / metabolism
  • Ovary / blood supply*
  • Ovary / metabolism*
  • Ovary / pathology*
  • Propanolamines / administration & dosage*
  • Rats
  • Rats, Wistar
  • Reactive Oxygen Species / metabolism
  • Reperfusion Injury / drug therapy*
  • Reperfusion Injury / etiology
  • Reperfusion Injury / metabolism
  • Reperfusion Injury / pathology
  • Superoxide Dismutase / metabolism
  • Torsion Abnormality / complications
  • bcl-2-Associated X Protein / metabolism


  • Adrenergic alpha-1 Receptor Antagonists
  • Bax protein, rat
  • Carbazoles
  • Propanolamines
  • Reactive Oxygen Species
  • bcl-2-Associated X Protein
  • Carvedilol
  • Malondialdehyde
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Casp3 protein, rat
  • Caspase 3