Formylpeptide Receptors Promote the Migration and Differentiation of Rat Neural Stem Cells

Sci Rep. 2016 May 13:6:25946. doi: 10.1038/srep25946.

Abstract

Neural stem cells (NSCs) bear characteristics for proliferation, migration and differentiation into three main neural cell type(s): neurons, astrocytes and/or oligodendrocytes. Formylpeptide receptors (Fprs), belonging to the family of G protein-coupled chemoattractant receptors, have been detected on neurons in the central nervous system (CNS). Here, we report that Fpr1 and Fpr2 are expressed on NSCs as detected with immunohistochemistry, RT-PCR and WB assays. In addition, Fpr1 and Fpr2 promoted NSC migration through F-actin polymerization and skewed NSC differentiation to neurons. Our study demonstrates a unique role of Fpr1 and Fpr2 in NSCs and opens a novel window for cell replacement therapies for brain and spinal cord injury.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism
  • Animals
  • Cell Differentiation
  • Cell Movement
  • Cell Proliferation
  • Cells, Cultured
  • Neural Stem Cells / cytology*
  • Neural Stem Cells / metabolism*
  • Rats
  • Receptors, Formyl Peptide / genetics
  • Receptors, Formyl Peptide / metabolism*
  • Receptors, Lipoxin / genetics
  • Receptors, Lipoxin / metabolism*

Substances

  • Actins
  • Fpr1 protein, rat
  • Receptors, Formyl Peptide
  • Receptors, Lipoxin
  • lipoxin A(4) receptor, rat