IgA production requires B cell interaction with subepithelial dendritic cells in Peyer's patches

Science. 2016 May 13;352(6287):aaf4822. doi: 10.1126/science.aaf4822.


Immunoglobulin A (IgA) induction primarily occurs in intestinal Peyer's patches (PPs). However, the cellular interactions necessary for IgA class switching are poorly defined. Here we show that in mice, activated B cells use the chemokine receptor CCR6 to access the subepithelial dome (SED) of PPs. There, B cells undergo prolonged interactions with SED dendritic cells (DCs). PP IgA class switching requires innate lymphoid cells, which promote lymphotoxin-β receptor (LTβR)-dependent maintenance of DCs. PP DCs augment IgA production by integrin αvβ8-mediated activation of transforming growth factor-β (TGFβ). In mice where B cells cannot access the SED, IgA responses against oral antigen and gut commensals are impaired. These studies establish the PP SED as a niche supporting DC-B cell interactions needed for TGFβ activation and induction of mucosal IgA responses.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / immunology*
  • Cell Communication / immunology
  • Cell Movement / immunology
  • Dendritic Cells / immunology*
  • Immunoglobulin A, Secretory / biosynthesis*
  • Immunoglobulin A, Secretory / genetics
  • Immunoglobulin Class Switching*
  • Integrins / immunology
  • Intestinal Mucosa / immunology
  • Lymphocyte Activation
  • Lymphotoxin beta Receptor / genetics
  • Lymphotoxin beta Receptor / immunology
  • Mice
  • Mice, Mutant Strains
  • Peyer's Patches / immunology*
  • Receptors, CCR6 / genetics
  • Receptors, CCR6 / immunology*


  • CCR6 protein, mouse
  • Immunoglobulin A, Secretory
  • Integrins
  • Lymphotoxin beta Receptor
  • Receptors, CCR6
  • integrin alphavbeta8